Usage costs
Because of the high costs for mass spectrometry, we need to charge the following amounts (in Euro).
Cost calculation for lipid measurements at the Lipidomics Facility of University of Bonn
Because of the high costs for mass spectrometry, we need to charge the following amounts (in Euro).
Please make sure that your samples are free of detergents, PEG, plasticizers etc. Also keep in mind that some organic solvents (e.g. chloroform) or some glass/plastic ware can contain minute amounts of lipids which can contaminate your sample. We will refrain from measuring such contaminated samples.
Info
We recommend that you extract your samples at your institution and ship them to us at ambient temperature.
We also recommend to have 5 samples (replicas) for each measuring point (wild type, mutant, etc.).
For large sample sets, a discount can be arranged
Costs are calculated for each sample processed
| A | Lipid isolation from cells/tissue | 20 Euro |
| B | Solid phase extraction (SPE) on silica columns | 20 Euro |
| C | Lipid quantification by direct infusion Q-TOF MS/MS | 50 Euro |
| D | Lipid quantification by LC-MS | 70 Euro |
| E | Fatty acid or sterol derivatization | 20 Euro |
| F | GC or GC-MS analysis | 20 Euro |
Examples:
The measurement of phospholipids/galactolipids of wild type and one mutant line of Arabidopsis plants, grown at normal and low temperature amounts to (you perform the lipid isolation)
2 lines x 5 replicas x 2 conditions x 50 Euro = 1000 Euro.
Measurement of triacylglycerol/diacylglycerol in 20 plant lines (we perform lipid isolation and SPE on silica columns)
20 lines x 5 replicas x (20 Euro + 20 Euro + 50 Euro) = 9000 Euro.
Contact for further questions
Peter Dörmann
Katharina Gutbrod
Helga Peisker